Dental amalgam is still the most widely used restorative material in the last 150 years especially in posterior teeth because of its high mechanical strength, durability, ease of manipulation, and low cost [1, 2, 3, 4, 5]. Dental amalgam is an alloy comprised of 50 % elemental mercury and a mixture of other metals such as silver, tin, copper, and sometimes palladium, indium and zinc [6, 7, 8]. Dental amalgam is considered as the primary source of continuous mercury exposure in general population [1, 9, 10, 11]. Mercury is a toxic element which can damage various organs such as central nervous system, renal, respiratory and hematologic systems [12, 13]. Because of the mercury toxicity, the use of mercury has been banned in some European countries [14]. The amount of mercury which releases from amalgam restorations depends on several factors such as number and size of the fillings, composition of amalgam, any other factors that causes load over the restorations like tooth brushing, chewing habits,and bruxism [8, 15].

Wi-Fi is a local area wireless computer networking technology and has been used drastically in houses and public places such as schools and hospitals during recent years [16]. It allows electronic devices such as personal computers, video-game consoles, smart phones, digital cameras and tablet computers to network using Institute of Electrical and Electronics Engineers (IEEE) 802.11 standards. These standards mainly use the 2.5 gigahertz (12 cm) UHF and 5 gigahertz (6 cm) SHF ISM radio bands [17]. The lower cost and easier deployment of these devices than wired computer networks lead to rapidly increase of Wi-Fi devices [18]. However, this also raised great public concern about the potential adverse effects of exposure to electromagnetic fields (EMFs) emitted from these devices [19].

The adverse health impacts associated to exposure to some common sources of electromagnetic fields including laptop computers, mobile phones, MRI and mobile phone jammers have been evaluated by our laboratory in our previous investigations [20, 21, 22, 23, 24]. To the best of our knowledge, this is the first study that evaluates the effect of exposure to Wi-Fi signals on mercury release from amalgam restorations.

Public concern about the possible adverse health effects of using Wi-Fi technology is increasing because of the widespread use of wireless communication systems [19]. In the present study, it was concluded that radiofrequency radiation emitted from Wi-Fi devices significantly increased mercury release from amalgam restorations.

It should be noted that the results obtained in the studies performed on the role of exposure to electromagnetic fields in magnetic resonance imaging on the microleakage of amalgam are strongly in line with the findings of Mortazavi et al. [29, 30]. To the best of our knowledge, our current study is the first study that investigates the effect of radiofrequency radiation emitted by Wi-Fi routers on mercury release from amalgam restorations.

Mercury is a toxic element which has adverse biological effects even at low doses [31]. Therefore, it seems to be necessary to apply a sensitive and reliable analytical technique to determine mercury content. Various analytical techniques has been used previously for the determination of mercury in environmental and biological samples such as cold vapor atomic absorption spectrometry (CVAAS), cold vapor fluorescence spectrometry (CVAFS), inductively coupled plasma optical emission spectrometry (ICP OES), electrothermal atomic absorption spectrometry (ET AAS), neutron activation analysis, mass spectrometry, anodic stripping voltammetry, and cold vapor inductively coupled plasma mass spectrometry (CV ICP-MS) [32, 33, 34, 35]. This study employed CVAAS method for measuring mercury released from dental amalgam. Because CVAAS is the most widely technique used in previous studies for detecting this element at low concentrations due to its high sensitivity and selectivity and because of its low cost [36, 37].

To improve the outcome of the west possible mercury release, we did not polish the cavities after restoration, because according to Ferracane et al. greater amounts of mercury would release from unpolished than polished surfaces [38].

Although the adverse health effects of the exposure to radiofrequency radiation emitted by Wi-Fi routers on some challenging phenomena such as human reproductive capabilities is well documented by some researchers around the world [39, 40], as far as we know, there is no report on the role of Wi-Fi radiation on the release of mercury from amalgam restorations. The mercury release from dental amalgam into saliva has been evaluated in previous studies both in vitro and in vivo conditions [25, 31, 41, 42, 43]. One of the limitation of in vivo studies, as Mortazavi et al. discussed in their study, was that the participants were referred by their own physicians and the investigators did not have control over the number and surface of amalgam fillings [41]. However, in our in vitro study, we could control these factors by using identical class V fillings with the same dimensions through application of a template during cavity preparations since the mercury exposure correlates significantly to the number and surface of fillings [8, 15]. We also could control some other confounding factors which differ inter individually such as chewing habits and thermal effects [15, 44]. On the other hand some factors that may decrease the mercury release such as the liberation of corrosive products by contact of food and bacteria did not also interference with our findings.

To the best of our knowledge, this is the first study which assesses the effect of exposure to Wi-Fi signals on mercury release from amalgam restorations. We speculated that exposure to radiofrequency emitted from Wi-Fi devices may result in mercury release from amalgam restorations. Further in vitro and in vivo studies are necessary to prove this contention.

CV ICP-MS, cold vapor inductively coupled plasma mass spectrometry; CVAAS, cold vapor atomic absorption spectrometry; CVAFS, cold vapor fluorescence spectrometry; EMF, electromagnetic fields; ET AAS, electrothermal atomic absorption spectrometry; ICP OES, inductively coupled plasma optical emission spectrometry; MRI, magnetic resonance imaging; SHF, super high frequency; UHF, ultra high frequency; Wi-Fi, wireless fidelity.